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super script iii reverse transcriptase kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher super script iii reverse transcriptase kit
    Super Script Iii Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/super+script+iii+reverse+transcriptase+system+kit/pmc11346181-331-10-16
    Average 86 stars, based on 1 article reviews
    super script iii reverse transcriptase kit - by Bioz Stars, 2026-11
    86/100 stars

    Images

    Related Articles

    Spectrophotometry:

    Article Title: The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons
    Article Snippet: RNA from gut tissues of rat was isolated using the Nucleo Spin RNA Triprep Kit or Clean up (Macherey-Nagel), respectively, according to the manufacturer's instructions. .. Potential genomic DNA contamination was removed by treatment with Turbo DNase (Ambion Inc), and RNA was quantified using an ND-1000 UV-Vis spectrophotometer (Nanodrop Technologies). cDNA was synthesized from 1 μg total RNA using the Super Script III Reverse Transcriptase System kit (Invitrogen) and diluted to a final concentration of 8 ng eq RNA/μl. qPCR was performed using StepOne Plus (Life Technologies) detection system with Fast SYBR Green (Life Technologies) master mix. ..

    Synthesized:

    Article Title: The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons
    Article Snippet: RNA from gut tissues of rat was isolated using the Nucleo Spin RNA Triprep Kit or Clean up (Macherey-Nagel), respectively, according to the manufacturer's instructions. .. Potential genomic DNA contamination was removed by treatment with Turbo DNase (Ambion Inc), and RNA was quantified using an ND-1000 UV-Vis spectrophotometer (Nanodrop Technologies). cDNA was synthesized from 1 μg total RNA using the Super Script III Reverse Transcriptase System kit (Invitrogen) and diluted to a final concentration of 8 ng eq RNA/μl. qPCR was performed using StepOne Plus (Life Technologies) detection system with Fast SYBR Green (Life Technologies) master mix. ..

    Article Title: Autolysis of Pichia pastoris induced by cold
    Article Snippet: Total RNA samples were treated with DNase I (Invitrogen) to remove DNA. .. The first DNA strand of cDNA encoding ENG was synthesized by reverse transcription from 1 μg total RNA using the Super Script III Reverse Transcriptase System Kit (Invitrogen). .. Primers and probes used for qRT-PCR analyses were designed by Applied Biosystems (Foster City, CA, USA; Table ).

    Reverse Transcription:

    Article Title: The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons
    Article Snippet: RNA from gut tissues of rat was isolated using the Nucleo Spin RNA Triprep Kit or Clean up (Macherey-Nagel), respectively, according to the manufacturer's instructions. .. Potential genomic DNA contamination was removed by treatment with Turbo DNase (Ambion Inc), and RNA was quantified using an ND-1000 UV-Vis spectrophotometer (Nanodrop Technologies). cDNA was synthesized from 1 μg total RNA using the Super Script III Reverse Transcriptase System kit (Invitrogen) and diluted to a final concentration of 8 ng eq RNA/μl. qPCR was performed using StepOne Plus (Life Technologies) detection system with Fast SYBR Green (Life Technologies) master mix. ..

    Article Title: Region-specific remodeling of the enteric nervous system and enteroendocrine cells in the colon of spinal cord injury patients.
    Article Snippet: Potential genomic DNA contamination was removed by treatment with Turbo DNase (Ambion Inc., USA), and RNA was quantified using a Nanodrop 200° ND-1000 UV–vis spectrophotometer (Nanodrop Technologies, USA). .. The reverse transcriptase reaction was performed using 1 μg total RNA incubated at 72 °C for 3 min using the Super Script III Reverse Transcriptase System kit (Thermo Fisher Scientific, France) in a Thermal Cycler 2720 thermocycler (Applied Biosystems, USA) (25 °C for 5 min, 50 °C for 55 min, 70 °C for 15 min). .. PCR amplification was performed using an Absolute Blue SYBR Green Fluorescein Kit (Roche Molecular Biochemicals, France) and run on a StepOnePlus system (Life Technologies).

    Article Title: Analysis of enteric nervous system and intestinal epithelial barrier to predict complications in Hirschsprung’s disease
    Article Snippet: .. The reverse transcriptase reaction was realised from 1 μg total RNA incubated at 72 °C for 3 min using the Super Script III Reverse Transcriptase System kit (THERMOFISHER) in the Thermal Cycler 2720 thermocycler (APPLIED BIOSYSTEM, Foster City, CA, USA) (25 °C for 5 min, 50 °C for 55 min, 70 °C for 15 min). ..

    Article Title: Region-specific remodeling of the enteric nervous system and enteroendocrine cells in the colon of spinal cord injury patients
    Article Snippet: Potential genomic DNA contamination was removed by treatment with Turbo DNase (Ambion Inc., USA), and RNA was quantified using a Nanodrop 200° ND-1000 UV–vis spectrophotometer (Nanodrop Technologies, USA). .. The reverse transcriptase reaction was performed using 1 μg total RNA incubated at 72 °C for 3 min using the Super Script III Reverse Transcriptase System kit (Thermo Fisher Scientific, France) in a Thermal Cycler 2720 thermocycler (Applied Biosystems, USA) (25 °C for 5 min, 50 °C for 55 min, 70 °C for 15 min). .. PCR amplification was performed using an Absolute Blue SYBR Green Fluorescein Kit (Roche Molecular Biochemicals, France) and run on a StepOnePlus system (Life Technologies).

    Article Title: Alterations of Prostanoid Expression and Intestinal Epithelial Barrier Functions in Ileus.
    Article Snippet: Introduction: Intestinal manipulation (IM)einduced inflammation could contribute to postoperative ileus (POI) pathophysiology via the modulation of prostanoid pathways.. To identify the prostanoids involved, we aimed to characterize the profile of prostanoids and their synthesis enzyme expression in a murine model of POI and to determine whether the altered prostanoids could contribute to POI.. Methods: Four or 14 h after IM in mice, gastrointestinal (GI) motility and intestinal epithelial barrier (IEB) permeability were assessed in vivo and ex vivo in Ussing chambers.

    Article Title: Autolysis of Pichia pastoris induced by cold
    Article Snippet: Total RNA samples were treated with DNase I (Invitrogen) to remove DNA. .. The first DNA strand of cDNA encoding ENG was synthesized by reverse transcription from 1 μg total RNA using the Super Script III Reverse Transcriptase System Kit (Invitrogen). .. Primers and probes used for qRT-PCR analyses were designed by Applied Biosystems (Foster City, CA, USA; Table ).

    Concentration Assay:

    Article Title: The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons
    Article Snippet: RNA from gut tissues of rat was isolated using the Nucleo Spin RNA Triprep Kit or Clean up (Macherey-Nagel), respectively, according to the manufacturer's instructions. .. Potential genomic DNA contamination was removed by treatment with Turbo DNase (Ambion Inc), and RNA was quantified using an ND-1000 UV-Vis spectrophotometer (Nanodrop Technologies). cDNA was synthesized from 1 μg total RNA using the Super Script III Reverse Transcriptase System kit (Invitrogen) and diluted to a final concentration of 8 ng eq RNA/μl. qPCR was performed using StepOne Plus (Life Technologies) detection system with Fast SYBR Green (Life Technologies) master mix. ..

    Real-time Polymerase Chain Reaction:

    Article Title: The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons
    Article Snippet: RNA from gut tissues of rat was isolated using the Nucleo Spin RNA Triprep Kit or Clean up (Macherey-Nagel), respectively, according to the manufacturer's instructions. .. Potential genomic DNA contamination was removed by treatment with Turbo DNase (Ambion Inc), and RNA was quantified using an ND-1000 UV-Vis spectrophotometer (Nanodrop Technologies). cDNA was synthesized from 1 μg total RNA using the Super Script III Reverse Transcriptase System kit (Invitrogen) and diluted to a final concentration of 8 ng eq RNA/μl. qPCR was performed using StepOne Plus (Life Technologies) detection system with Fast SYBR Green (Life Technologies) master mix. ..

    SYBR Green Assay:

    Article Title: The ephrin receptor EphB2 regulates the connectivity and activity of enteric neurons
    Article Snippet: RNA from gut tissues of rat was isolated using the Nucleo Spin RNA Triprep Kit or Clean up (Macherey-Nagel), respectively, according to the manufacturer's instructions. .. Potential genomic DNA contamination was removed by treatment with Turbo DNase (Ambion Inc), and RNA was quantified using an ND-1000 UV-Vis spectrophotometer (Nanodrop Technologies). cDNA was synthesized from 1 μg total RNA using the Super Script III Reverse Transcriptase System kit (Invitrogen) and diluted to a final concentration of 8 ng eq RNA/μl. qPCR was performed using StepOne Plus (Life Technologies) detection system with Fast SYBR Green (Life Technologies) master mix. ..

    Incubation:

    Article Title: Region-specific remodeling of the enteric nervous system and enteroendocrine cells in the colon of spinal cord injury patients.
    Article Snippet: Potential genomic DNA contamination was removed by treatment with Turbo DNase (Ambion Inc., USA), and RNA was quantified using a Nanodrop 200° ND-1000 UV–vis spectrophotometer (Nanodrop Technologies, USA). .. The reverse transcriptase reaction was performed using 1 μg total RNA incubated at 72 °C for 3 min using the Super Script III Reverse Transcriptase System kit (Thermo Fisher Scientific, France) in a Thermal Cycler 2720 thermocycler (Applied Biosystems, USA) (25 °C for 5 min, 50 °C for 55 min, 70 °C for 15 min). .. PCR amplification was performed using an Absolute Blue SYBR Green Fluorescein Kit (Roche Molecular Biochemicals, France) and run on a StepOnePlus system (Life Technologies).

    Article Title: Analysis of enteric nervous system and intestinal epithelial barrier to predict complications in Hirschsprung’s disease
    Article Snippet: .. The reverse transcriptase reaction was realised from 1 μg total RNA incubated at 72 °C for 3 min using the Super Script III Reverse Transcriptase System kit (THERMOFISHER) in the Thermal Cycler 2720 thermocycler (APPLIED BIOSYSTEM, Foster City, CA, USA) (25 °C for 5 min, 50 °C for 55 min, 70 °C for 15 min). ..

    Article Title: Region-specific remodeling of the enteric nervous system and enteroendocrine cells in the colon of spinal cord injury patients
    Article Snippet: Potential genomic DNA contamination was removed by treatment with Turbo DNase (Ambion Inc., USA), and RNA was quantified using a Nanodrop 200° ND-1000 UV–vis spectrophotometer (Nanodrop Technologies, USA). .. The reverse transcriptase reaction was performed using 1 μg total RNA incubated at 72 °C for 3 min using the Super Script III Reverse Transcriptase System kit (Thermo Fisher Scientific, France) in a Thermal Cycler 2720 thermocycler (Applied Biosystems, USA) (25 °C for 5 min, 50 °C for 55 min, 70 °C for 15 min). .. PCR amplification was performed using an Absolute Blue SYBR Green Fluorescein Kit (Roche Molecular Biochemicals, France) and run on a StepOnePlus system (Life Technologies).

    Article Title: Alterations of Prostanoid Expression and Intestinal Epithelial Barrier Functions in Ileus.
    Article Snippet: Introduction: Intestinal manipulation (IM)einduced inflammation could contribute to postoperative ileus (POI) pathophysiology via the modulation of prostanoid pathways.. To identify the prostanoids involved, we aimed to characterize the profile of prostanoids and their synthesis enzyme expression in a murine model of POI and to determine whether the altered prostanoids could contribute to POI.. Methods: Four or 14 h after IM in mice, gastrointestinal (GI) motility and intestinal epithelial barrier (IEB) permeability were assessed in vivo and ex vivo in Ussing chambers.



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